dSCOPE is a web server developed for detecting sequences critical for phase separation related proteins. Based on random forest algorithm, the predictor achieved promising performances with three bright spots as follows :ⅰ) Multiple features are extracted to build the prediction model; ⅱ) Various sequence and structural properties including phase separation region, disorder, exposed & buried, polar, low complexity region, charge, secondary structure, prion-like region, surface accessibility, hydropathy, subcellular location are visualized; ⅲ) Pre-predicted key regions of phase separation for human proteome are provided.

ROC for cross validation (left), ROC for dSCOPE compared to other features, including IUPred and PLAAC(right).

Web server procedure

Step1: Paste your FASTA format sequence into the input textarea, or you can click the example button to run the default sequence.

Step2: Choose a kind of organism, along with a threshold, and click submit.

Web server result

1. The dSCOPE prediction results including the phase separation region, the average dSCOPE score, the threshold and the peptide.

2. you can arrange the result table, and we only display the first five and the last five amino acids of the peptide, you can see the full sequence if you click it.

3. We dispaly the predicted phase separation region, the dSCOPE score of every amino acid, the structural characteristics (disorder, exposed or buried, polar, low complex, charge, second structure, prion-like, surface accessibility and hydropathy), and the subcellular location.

4. You can scale all the graphics by control element in the top, or through the mouse wheel, the detailed information will be shown if you hover the mouse on an amino acid.

Database search procedure

Step1: You can choose a search type and enter a keyword to start searching the dSCOPE, and we provide an example for each search type, including UniProt ID, gene name and protein name.

Step2: All matched results will be displayed in a table at the bottom of the search page, you can click the more button to see the predicted result of the protein.

Database search result

1. The dSCOPE prediction results including the phase separation region, the average dSCOPE score, the threshold and the peptide.

2. you can arrange the result table, and we only display the first five and the last five amino acids of the peptide, you can see the full sequence if you click it.

3. We collected the information of the protein from UniProt, and a corresponding link is provided.

4. We dispaly the predicted phase separation region, the dSCOPE score of every amino acid, the structural characteristics (disorder, exposed or buried, polar, low complex, charge, second structure, prion-like, surface accessibility and hydropathy), and the subcellular location.

5. You can scale all the graphics by control element in the top, or through the mouse wheel, the detailed information will be shown if you hover the mouse on an amino acid.